THE ROLE OF ADSORPTION IN CONTROLLING THE RATE OF REACTION OF CHLORAMBUCIL WITH PROTEIN
- 1 April 1963
- journal article
- Published by Canadian Science Publishing in Canadian Journal of Biochemistry and Physiology
- Vol. 41 (4) , 931-939
- https://doi.org/10.1139/o63-105
Abstract
Two proteins, haemoglobin and bovine serum albumin, have been studied with respect to their rates of alkylation by chlorambucil in vitro at 37 °C and pH 8.4. The proteins are of nearly the same molecular weight and free carboxylic acid content, but the alkylation reaction is 30 times faster with haemoglobin. On the other hand, the adsorption of chlorambucil by albumin is 20 times greater than that exhibited by haemoglobin. This inverse relationship between extent of adsorption and reaction rate suggests that adsorption protects the chlorambucil from activation in the solvent.Keywords
This publication has 6 references indexed in Scilit:
- The recovery of free chlorambucil from solution in blood serumBiochemical Pharmacology, 1962
- THE CONSTRUCTION OF AN ULTRAFILTRATION UNIT TO MEASURE THE BINDING OF CERTAIN NITROGEN MUSTARDS TO SERUM PROTEINCanadian Journal of Biochemistry and Physiology, 1962
- Some interactions of nitrogen mustards with constituents of human blood serumBiochemical Pharmacology, 1961
- Die Konstitution des normalen adulten HumanhämoglobinsHoppe-Seyler´s Zeitschrift Für Physiologische Chemie, 1961
- The reactivity of radiomimetic compounds II: Sites of reaction of nitrogen mustards, epoxides, methane sulphonic acid esters and an ethyleneimine with bovine serum albuminBiochemical Pharmacology, 1958
- THE REACTIONS OF THE “RADIOMIMETIC” ALKYLATING AGENTS WITH MACROMOLECULES IN VITROAnnals of the New York Academy of Sciences, 1958