Effect of Silibinin on the Activity and Expression of Superoxide Dismutase in Lymphocytes from Patients with Chronic Alcoholic Liver Disease
- 1 January 1987
- journal article
- Published by Taylor & Francis in Free Radical Research Communications
- Vol. 3 (6) , 373-377
- https://doi.org/10.3109/10715768709088078
Abstract
The in vitro and in vivo effects of the naturally occuring flavolignan hepatoprotective agent silibinin on the expression and activity of superoxide dismutase (SOD) enzyme were studied in lymphocytes from patients with chronic alcoholic liver disease. In vitro incubation with silibinin in a concentration corresponding to the usual therapeutic dosage markedly increased the SOD--expression of lymphocytes as measured by flow-cytofluorimetry following staining with monoclonal anti-Cu, Zn-SOD--antibody and FITC-conjugated anti-mouse Ig. In vivo treatment with the drug restored the originally low SOD activity of the patients' lymphocytes. These data indirectly suggest that antioxidant activity might be one of the important factors in the hepatoprotective action of silibinin.Keywords
This publication has 10 references indexed in Scilit:
- Changes of the Serum Superoxide Dismutase Content in Gramoxone Poisoned Patients, Measured by Anti-SOD Monoclonal AntibodyPublished by Springer Nature ,1985
- Liver superoxide dismutases and catalase during ethanol inhalation and withdrawalPharmacology Biochemistry and Behavior, 1983
- Depressed hepatic glutathione and increased diene conjugates in alcoholic liver diseaseDigestive Diseases and Sciences, 1983
- Halothane Hepatotoxicity: Relation Between Metabolic Activation, Hypoxia, Covalent Binding, Lipid Peroxidation and Liver Cell DamageHepatology, 1983
- ROLE OF VITAMIN E IN THE PREVENTION OF HEPATOCELLULAR DAMAGE: CLINICAL AND EXPERIMENTAL APPROACHAnnals of the New York Academy of Sciences, 1982
- Free-Radical Damage in LiverPublished by Elsevier ,1980
- Silymarin, an inhibitor of lipoxygenaseCellular and Molecular Life Sciences, 1979
- Comparative evaluation of antiperoxidative action of silymarin and other flavonoidsPharmacological Research Communications, 1978
- Inhibitory action of silymarin of lipid peroxide formation in rat liver mitochondria and microsomesBiochemical Pharmacology, 1977
- Mechanism of the Stimulation of RNA Synthesis in Rat Liver Nuclei by SilybinHoppe-Seyler´s Zeitschrift Für Physiologische Chemie, 1977