Amplification of the c‐erbB‐2 gene detected by FISH in gastric cancers

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Abstract
The amplification and overexpression of the c‐erbB‐2 gene are considered to be Implicated In the process of carcincogenesis of a variety of human tumors. The amplification and overexpression of c‐erbB‐2 were investigated in 48 surgically resected human gastric cancers by means of fluorescence In situ hybridization and immunohistochemistry. DNA ploidy was determined by flow cytometry. The c‐erbB‐2 amplification was demonstrated as a cluster of signals, suggesting homogeneously staining region (HSR), in three tumors (6.3%) accompanied by the overexpression of its protein. Such overexpression was detected In another tumor without amplification of the c‐erbB‐2 gene. All tumors with amplification and overexpression of c‐erbB‐2 were differentiated adenocarcinoma histologically, but only 10.3 and 13.8% of differentiated carcinomas showed amplification and over‐expression of the c‐erbB‐2 gene, respectively. There was no relationship between the amplification and overexpression of c‐erbB‐2 and the depth of tumor invaslon and lymph node Involvement. Three of four cases with overexpression of c‐erbB‐2 were classified Into DNA aneupldd tumor.