NF‐κB‐mediated regulation of urokinase gene expression by PMA and TNF‐α in human A549 cells
Open Access
- 9 September 1996
- journal article
- Published by Wiley in FEBS Letters
- Vol. 393 (1) , 69-73
- https://doi.org/10.1016/0014-5793(96)00854-x
Abstract
Binding sites for different transcription factors have been identified in the regulatory region of the human uPA gene. We investigated the role of NF‐κB and AP‐1 families of transcription factors in the induction of uPA mRNA by TNF‐α and PMA in the A549 cell line constitutively expressing uPA mRNA and protein. Using the protein synthesis inhibitor cycloheximide and the antioxidant PDTC, that have an opposite effect on NF‐κB and AP‐1 activation, we showed that uPA mRNA induction by TNF‐α and PMA in the A549 cell line is mainly due to NF‐κB activation.Keywords
This publication has 22 references indexed in Scilit:
- Inhibition of NF-KB-Rel A expression by antisense oligodeoxynucleotides suppresses synthesis of urokinase-type plasminogen activator (uPA) but not its inhibitor PAl-1Nucleic Acids Research, 1995
- Purification, reconstitution, and I kappa B association of the c-Rel-p65 (RelA) complex, a strong activator of transcription.Molecular and Cellular Biology, 1994
- Physiological consequences of loss of plasminogen activator gene function in miceNature, 1994
- Urokinase-type plasminogen activator and its receptor: new targets for anti-metastatic therapy?Trends in Pharmacological Sciences, 1994
- Rapid proteolysis of IκB-α is necessary for activation of transcription factor NF-κBNature, 1993
- IMF-κB and Rel: Participants in a Multiform Transcriptional Regulatory SystemPublished by Elsevier ,1993
- Tumor Necrosis Factor Stimulates Urokinase-Type Plasminogen Activator and Inhibitor Type 1 Production in A549 Lung Carcinoma Cells: Treatment of Monolayer and Tridimensional CulturesSeminars in Thrombosis and Hemostasis, 1991
- Cloning of the DNA-binding subunit of human nuclear factor kappa B: the level of its mRNA is strongly regulated by phorbol ester or tumor necrosis factor alpha.Proceedings of the National Academy of Sciences, 1991
- Epidermal growth factor stimulates transcription of the c-jun proto-oncogene in rat fibroblastsNature, 1988
- An inducible transcription factor activates expression of human immunodeficiency virus in T cellsNature, 1987