Molecular analysis ofShigella boydiiO1 O-antigen gene cluster and its PCR typing
- 1 May 2005
- journal article
- Published by Canadian Science Publishing in Canadian Journal of Microbiology
- Vol. 51 (5) , 387-392
- https://doi.org/10.1139/w05-015
Abstract
Shigella is an important human pathogen and is closely related to Escherichia coli. O-antigen is the most variable part of the lipopolysaccharide on the cell surface of Gram-negative bacteria and plays an important role in pathogenicity. The O-antigen gene cluster of S. boydii O1 was sequenced. The putative genes encoding enzymes for rhamnose synthesis, transferases, O-unit flippase, and O-unit polymerase were identified on the basis of homology. The O-antigen gene clusters of S. boydii O1 and E. coli O149, which share the same O-antigen form, were found to have the same genes and organization by adjacent gene PCR assay. Two genes specific for S. boydii O1 and E. coli O149 were identified by PCR screening against E. coli- and Shigella-type strains of the 186 known O-antigen forms and 39 E. coli clinical isolates. A PCR sensitivity of 103to 104CFU/mL overnight culture of S. boydii O1 and E. coli O149 was obtained. S. boydii O1 and E. coli O149 were differentiated by PCR using lacZ- and cadA-based primers.Key words: O-antigen gene cluster, S. boydii O1, E. coli O149, molecular typing.Keywords
This publication has 26 references indexed in Scilit:
- Identification of Escherichia coli O114 O-Antigen Gene Cluster and Development of an O114 Serogroup-Specific PCR AssayJournal of Clinical Microbiology, 2004
- Extensive Genomic Diversity in Pathogenic Escherichia coli and Shigella Strains Revealed by Comparative Genomic Hybridization MicroarrayJournal of Bacteriology, 2004
- Identification of Escherichia coli O172 O-antigen gene cluster and development of a serogroup-specific PCR assayJournal of Applied Microbiology, 2004
- Detection of Escherichia coli Serogroups O26 and O113 by PCR Amplification of the wzx and wzy GenesApplied and Environmental Microbiology, 2004
- Structural and Genetic Characterization of the Shigella boydii Type 13 O AntigenJournal of Bacteriology, 2004
- CadC Is the Preferential Target of a Convergent Evolution Driving Enteroinvasive Escherichia coli toward a Lysine Decarboxylase-Defective PhenotypeInfection and Immunity, 2003
- Sequence of the Escherichia coli O121 O-Antigen Gene Cluster and Detection of Enterohemorrhagic E. coli O121 by PCR Amplification of the wzx and wzy GenesJournal of Clinical Microbiology, 2003
- Pathoadaptive Mutations That Enhance Virulence: Genetic Organization of the cadA Regions of Shigella sppInfection and Immunity, 2001
- Role of the rfe gene in the biosynthesis of the Escherichia coli O7-specific lipopolysaccharide and other O-specific polysaccharides containing N-acetylglucosamineJournal of Bacteriology, 1994
- Structural studies of the Escherichia coli O-149 O-antigen polysaccharideCarbohydrate Research, 1988