Conservation of binding site specificity of three yeast DNA binding proteins
- 24 February 1992
- journal article
- Published by Wiley in FEBS Letters
- Vol. 298 (2-3) , 203-205
- https://doi.org/10.1016/0014-5793(92)80057-n
Abstract
Sequence specific binding of protein extracts from 13 different yeast species to three oligonucleotide probes and two points mutants derived from Saccharomyces cerevisiae DNA binding proteins were tested using mobility shift assays. The probes were high affinity binding sites for GRF1/RAP1/ ABF1 and CP1/CPF1. Most yeasts in the genus Saccharomyces showed specific binding to all three probes and also displayed similar sequence requirements when challenged by molar excesses of mutant probes. The affinities for the probes varied amongst the other yeasts tested, but in general, CPF1 binding activity was the most widespread, while the other two were more limited.Keywords
This publication has 17 references indexed in Scilit:
- Multi-functional DNA proteins in yeast: The factors GFI and GFII are identical to the ARS-binding factor ABFI and the centromere-binding factor CPF1 respectivelyYeast, 1991
- Phylogenetic relationships among species of Saccharomyces, Schizosaccharomyces, Debaryomyces and Schwanniomyces determined from partial ribosomal RNA sequencesYeast, 1991
- Regulatory DNA‐binding proteins in yeast: An overviewYeast, 1990
- Yeast general transcription factor GFI: sequence requirements for binding to DNA and evolutionary conservationNucleic Acids Research, 1990
- A yeast ARS-binding protein activates transcription synergistically in combination with other weak activating factors.Molecular and Cellular Biology, 1990
- Connections between transcriptional activators, silencers, and telomeres as revealed by functional analysis of a yeast DNA-binding protein.Molecular and Cellular Biology, 1988
- Two DNA-binding factors recognize specific sequences at silencers, upstream activating sequences, autonomously replicating sequences, and telomeres in Saccharomyces cerevisiae.Molecular and Cellular Biology, 1988
- The value of orthogonal-field alternation gel electrophoresis as a rapid identification process for some species representing the genus SaccharomycesJournal of Microbiological Methods, 1987
- Purification and cloning of a DNA binding protein from yeast that binds to both silencer and activator elementsCell, 1987
- Deoxyribonucleic Acid Relatedness among Species of the Genus Saccharomyces Sensu StrictoInternational Journal of Systematic and Evolutionary Microbiology, 1985