A bacterial protein requirement for the bacteriophage λ terminase reaction
Open Access
- 1 January 1986
- journal article
- Published by Oxford University Press (OUP) in Nucleic Acids Research
- Vol. 14 (24) , 9797-9809
- https://doi.org/10.1093/nar/14.24.9797
Abstract
The bacteriophage lambda torminase enzyme cleaves the cohesive-end sites of λ DNA to yield the protruding 5'-termini of the mature molecule. In vitro, this ondonnclcolytic event requires a protein factor which has been isolated and purified from extracts of uninfected E. Coli. The terminase host factor (THF) is a heat stable basic protein of M.W. approximately 22, 000. The integration host factor (HIF) protein of E. Coli can efficiently substitute for THF in the terminase reaction; however, THF can be demonstrated to be physically present in, and isolated with full biological activity from extracts of cells defective or deficient in IHF.Keywords
This publication has 25 references indexed in Scilit:
- G inversion in bacteriophage Mu DNA is stimulated by a site within the invertase gene and a host factorCell, 1985
- Essential interaction between lambdoid phage 21 terminase and the Escherichia coli integrative host factorJournal of Molecular Biology, 1985
- E. coli integration host factor binds to specific sites in DNACell, 1984
- Nucleotide sequence of bacteriophage λ DNAJournal of Molecular Biology, 1982
- Regulation of bacteriophage λ int gene expressionJournal of Molecular Biology, 1982
- INTEGRATION AND EXCISION OF BACTERIOPHAGE λ: THE MECHANISM OF CONSERVATIVE SITE SPECIFIC RECOMBINATIONAnnual Review of Genetics, 1981
- int-h: an int mutation of phage λ that enhances site-specific recombinationCell, 1980
- Am E. coli gene product required for λ site-specific recombinationCell, 1980
- High resolution two-dimensional electrophoresis of basic as well as acidic proteinsCell, 1977
- Interaction of DNA with DNA‐Binding ProteinsEuropean Journal of Biochemistry, 1976