Identification and Chromosome Assignment of a Human Gene Encoding a Novel Phosphatidylinositol-3 kinase

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Abstract
We identified a novel phosphatidylinositol (PI) 3-kinase by screening human brain cDNA libraries with probes designed from the conserved kinase-domain sequence. Analysis of cDNAs indicated that two different forms of transcripts are present: one is the full-length form composedof 1,044 amino acid residues andthe other is the short form that the N-terminal 216 amino acid residues including a putative p85 binding domain has been truncated(828 amino acid residues). Database search revealedthe sequence of the full-length form to be identical to that recently registered by D. Chantry et al. (Accession No. U86453 in GenBank release, August 1997). Northern blot analysis showedthis mRNA to be ubiquitously expressed in various tissues, with relatively higher expression was observedin spleen, thymus and leukocytes. Based on fluorescence in situ hybridization and PCR-based analyses with both human/rodent mono-chromosomal hybrid cell panels andrad iation hybridmapping panels, this gene was localizedto chromosome region 1p36.2. This region is frequently lost in a variety of human malignancies, including neuroblastoma. The novel PI3K could be a candidate target of the 1p36 alteration that occurs in neuroendocrine tumors.

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