A western blot to detect antibody to avian reovirus

Abstract
Purified reoviruses RAM‐1 and 74/2 were combined and used as antigen. The Western blot detected antibodies to 16/16 avian reoviruses in sera from experimentally inoculated chickens, and to 6/7 reoviruses in contact sera from naturally infected chickens. The test was specific for avian reovirus and was more sensitive and specific than immunofluorescence.