The state of phosphorylation in vivo of membrane-bound phosphoproteins in rat brain
- 1 June 1973
- journal article
- Published by Portland Press Ltd. in Biochemical Journal
- Vol. 133 (2) , 387-389
- https://doi.org/10.1042/bj1330387
Abstract
The alkali-labile P content of membrane protein prepared from rapidly frozen rat brain was measured, CuSO4 being used to inhibit protein phosphatase activity during subcellular fractionation. The P content of the membrane fraction was significantly increased (+12%) over the control value by incubation of homogenates with ATP before fractionation. This suggests that the membrane protein in rat brain is normally only partially phosphorylated.This publication has 0 references indexed in Scilit: