Conformational Changes of 4-Carboxyglutamic Acid-Containing Protein from Bovine Bone by Binding of Alkaline Earth Ions
- 1 January 1983
- journal article
- research article
- Published by Walter de Gruyter GmbH in Hoppe-Seyler´s Zeitschrift Für Physiologische Chemie
- Vol. 364 (1) , 31-40
- https://doi.org/10.1515/bchm2.1983.364.1.31
Abstract
The effect of Mg2+, Ca2+ and Sr2+ on bovine bone Gla [4 carboxyglutamic-acid-containing] protein were studied by CD [circular dichroism] spectroscopy and UV difference spectroscopy; binding of Mg2+ and Ca2+ was studied by equilibrium dialysis. CD measurements indicate an increase of .alpha.-helix upon addition of alkaline earth ions. UV difference spectra show a change in the environment of a tyrosine residue. Evaluation of the results of all 3 studies indicates positive cooperativity in binding of Ca2+ and Sr2+ and negative or no cooperativity in binding of Mg2+.This publication has 21 references indexed in Scilit:
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