Specific Activities of Seven Urinary Metabolites of Estradiol-17β-6, 7-3H in Pregnant Women

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Abstract
Estradiol-17[beta]-6,7-H3 was injected into 4 women in late pregnancy (33 weeks-term) and 7 labeled metabolites were purified from the urine. These were ace-tylated with acetic anhydride-1-C14 and the acetates were purified by repeated crystallization with unlabeled carrier to constant isotope ratio. The specific activities of the H3-labeled metabolites were calculated from the H3: C14 ratios and were found to be (cmp/[mu]g): estrone 38-65, estradiol-17[beta] 45-77, 16[alpha]-hydroxy estrone 24-44, 16-ketoestradiol-17[beta] 25-36, 16-epiestriol 22-36, estriol 6-8. That of 2-methoxyestrone was not similar to that of estriol. These results may indicate that the endogenous 16-oxygenated metabolites other than estriol are mainly maternal conversion products of secreted estrone or estradiol-17[beta], with perhaps some part arising through conversion in a fetal compartment.

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