Chromosome analysis of isolated colony erythroblasts in chronic myelogenous leukaemia

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Abstract
To isolate and karyotype the progeny of erythroid progenitors, colony erythroplasts derived from plasma clot marrow cultures from 2 healthy adults and 2 patients with newly diagnosed Ph1+ chronic myelogenous leukemia (CML) were applied to discontinuous Stractan density gradients. Erythroid colony proliferation by patient cells was increased relative to that of normal donor cells (P < 0.01). Endogenous colonies appeared in patient but not in normal donor marrow cultures. Greater than 95% of nucleated cells equilibrating at P .gtoreq. 1.071 were basophilic proerythroblasts. While analysis of chromosome spreads of normal donor cells in this fraction showed normal karyotypes, cells from patient marrow cultures were Ph1+, whether cultured in the presence or absence of added erythropoietin. Chromosome abnormalities of erythroid progenitors may be expressed by their progeny in tissue culture. Stractan may be a useful supporting medium for separating colony erythroblasts for chromosome analysis.