N.m.r. and conformational studies of some 1,4-linked disaccharides

Abstract
1 H and 13C N.m.r. studies and conformational analysis have been performed on eight 1,4-linked disaccharides in which the glycosidic linkages are in different stereochemical environments. The disaccharide glycosides have been divided into two groups, one containing α-DD-, β-LD-, and β-DL-glycosides, and one containing β-DD-, α-LD-, and α-DL-glycosides with typical chemical shift differences for each group. The conformational analysis, using the HSEA-approach, indicates a number of proton–oxygen and proton–proton interactions resulting in downfield and upfield shifts of the proton signals, respectively. The 13C n.m.r. glycosylation shifts obtained have been used to simulate spectra of polysaccharides containing 1,4-linkages.

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