Isolation of a cDNA encoding the largest subunit of TFIIA reveals functions important for activated transcription.
Open Access
- 1 November 1993
- journal article
- research article
- Published by Cold Spring Harbor Laboratory in Genes & Development
- Vol. 7 (11) , 2246-2257
- https://doi.org/10.1101/gad.7.11.2246
Abstract
Transcription factor IIA has been shown to interact with the TATA-binding protein and to act early during preinitiation complex formation. The human factor is composed of three subunits (alpha, beta, gamma). A human cDNA clone encoding the largest subunit of TFIIA (alpha) was isolated. The recombinant alpha polypeptide, together with the beta and gamma subunits, was capable of reconstituting TFIIA activity. Studies using antibodies raised against recombinant alpha polypeptide demonstrate that TFIIA can be an integral component of the preinitiation complex. We demonstrate that TFIIA not only interacts with TBP but also can associate with the TFIID complex. Functional assays establish that TFIIA has no apparent role in basal transcription but plays an important role in activation of transcription. Interestingly, amino acid sequence analyses of the beta-subunit demonstrate these residues to be entirely contained within the carboxyl terminus of the cDNA clone encoding the alpha-subunit.Keywords
This publication has 47 references indexed in Scilit:
- Multiple functional domains of human transcription factor IIB: distinct interactions with two general transcription factors and RNA polymerase II.Genes & Development, 1993
- Drosophila 230-kD TFIID subunit, a functional homolog of the human cell cycle gene product, negatively regulates DNA binding of the TATA box-binding subunit of TFIID.Genes & Development, 1993
- An ATP-dependent inhibitor of TBP binding to DNA.Genes & Development, 1993
- The p250 subunit of native TATA box-binding factor TFIID is the cell-cycle regulatory protein CCG1Nature, 1993
- Dr1, a TATA-binding protein-associated phosphoprotein and inhibitor of class II gene transcriptionCell, 1992
- Advances in RNA polymerase II transcriptionCurrent Opinion in Cell Biology, 1992
- Transcription Factor IID Mutants Defective for Interaction with Transcription Factor IIAScience, 1992
- Structure and functional properties of human general transcription factor IIENature, 1991
- Control of c-Jun activity by interaction of a cell-specific inhibitor with regulatory domain δ: Differences between v- and c-JunCell, 1990
- Mechanism of transcriptional activation by Sp1: Evidence for coactivatorsCell, 1990