PSEUDORABIES VIRUS, PORCINE PARVOVIRUS, AND PORCINE ENTEROVIRUS INTERACTIONS WITH THE ZONA PELLUCIDA OF THE PORCINE EMBRYO

  • 1 January 1983
    • journal article
    • research article
    • Vol. 44  (6) , 1036-1039
Abstract
Porcine embryos (93) were incubated on cell monolayers that had been previously inoculated with pseudorabies virus (PV), porcine parvovirus (PPV), or both of 2 porcine enteroviruses. After 2, 24 or 48 h of incubation, the embryos were fixed in glutaraldehyde and examined by EM. PV adsorbed to the zona pellucida (ZP) and entered sperm tracks in the ZP. The PPV and both enteroviruses entered pores in the ZP and were associated with sperm that were at or near the outer surface of the ZP. PPV was seen enmeshed in cellular debris on the outer surface of the ZP. Evidence of a productive viral infection of the blastomeres of the embryos was not found.