Collisionally‐activated dissociation spectra of linear peptides in matrix‐assisted laser desorption/ionization time‐of‐flight mass spectrometry
- 1 January 1995
- journal article
- research article
- Published by Wiley in Rapid Communications in Mass Spectrometry
- Vol. 9 (14) , 1342-1344
- https://doi.org/10.1002/rcm.1290091404
Abstract
Collisionally‐activated dissociation (CAD) studies of linear peptides were performed using matrix‐assisted laser desorption/ionization time‐of‐flight mass spectrometry. Four different gases, viz. helium, nitrogen, argon and xenon, were introduced into a gas cell, installed close to the ion source of TOF mass spectrometer, to obtain high‐energy CAD information. The CAD spectral patterns for the linear peptides obtained using the four gases were almost the same as each other. The fragments characteristically obtained in conventional high‐energy CAD using tandem mass spectrometry were observed also in these spectra. A difference between CAD spectra and post‐source decay product‐ion spectra has been demonstrated.Keywords
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