NEW DATA ON STRUCTURE AND BUDDING OF FV3 (FROG VIRUS-3)

  • 1 January 1975
    • journal article
    • research article
    • Vol. B126  (4) , 447-+
Abstract
Freeze-etchings of FV3 suspensions revealed the perfectly icosahedral structure of the viral nucleocapsids. A fine substructure suggesting the existence of numerous subunits of small dimensions was detected at their surface. Replicas of infected [chick embryo fibroblast] cells obtained by freeze-etching revealed modifications of the external face of the cytoplasmic membrane occuring at the level of the budding viral particles; in these zones parallel stripes appeared, whose elementary structure is constant (13.5 nm)/ Scanning EM of the infected cells revealed spreading of the budding process on all the cells of the same culture. By counting the number of budding particles per square micron it was possible to demonstrate the heterogeneity of the budding process at the surface of the cells.

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