Diauxic growth of the fission yeast Schizosaccharomyces pombe in mixtures of D-glucose and ethanol or acetate

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Abstract
The fission yeast Schizosaccharomyces pombe was unable to utilize ethanol or acetate as its sole carbon source for growth. However, ethanol and acetate were utilized in the presence of D-glucose during diauxic growth. No mutants capable of utilizing ethanol or acetate as sole carbon source were isolated from cultures grown in glucose together with ethanol or acetate. Low concentrations of acetate facilitated growth with glucose, whereas high concentrations of ethanol or acetate were inhibitory. Growing cells readily took up [1-14C]ethanol and [1-14C]acetate. The exogenous [1-14C]acetate was initially incorporated into biomacromolecules which were subsequently catabolized.

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