A Rapid Radioimmunoassay Using 125I-Labeled Staphylococcal Protein A for Antibody to Varicella-Zoster Virus

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Abstract
A sensitive radioimmunoassay for serum antibody to varicella-zoster virus is described; it uses 125I-Iabeled staphylococcal protein A and a specially designed immunofiltration apparatus. The assay accurately distinguishes between individuals who are susceptible and those who are immune to infection with varicella-zoster virus. In addition, it can detect passive antibody in recipients of varicella-zoster immune globulin. This radioimmunoassay also detects the heterologous antibody responses that occasionally occur in patients infected with herpes simplex virus, which also have been detected by other antibody assays. The particular advantages of this assay are the use of noninfectious reagents, the speed of execution <3 hr), the requirement for only small quantities of serum (30 μl), the objectivity of end-point determination, and the capability of screening large numbers of sera. Consequently, this radioimmunoassay is especially useful for the rapid identification of susceptible individuals, which is essential for the appropriate management of patients and hospital personnel after exposure to varicella.