Use of New HPLC Resins to Solve Old Problems

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Abstract
A summation of recent experiences from this laboratory is described in this report. In the case of isolating peptides for eventual use in protein sequence analysis, an argument is made for the return to multistep chromatography in order to simplify complex mixtures of peptide fragments. Making use of new resins for ion exchange and reverse phase chromatography, yield of material even after three chromatographic steps is in excess of 90%. A partial enzymatic digest yielding multiple overlapping peptides can readily be sorted by this approach. For the isolation of monoclonal antibodies, the use of carboxymethyl derivatized synthetic resins allows for rapid isolation and concentration of antibodies which can then be highly purified on diethylaminoethyl polymeric based resins.

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