Interaction of the CRP-cAMP complex with the cea regulatory region
- 1 February 1989
- journal article
- research article
- Published by Springer Nature in Molecular Genetics and Genomics
- Vol. 215 (3) , 537-542
- https://doi.org/10.1007/bf00427053
Abstract
Analysis of the induction of expression of cea-lacZ fusions in cya and crp mutants showed that catabolite repression affects the kinetics of induction and the rate of induced synthesis. In a cya mutant, addition of cAMP reduced the induction lag and increased the amount of β-galactosidase produced. The CRP-cAMP complex was found to bind to two sites 5′ to the cea promoter, but deletion analysis showed that only one of these was involved in the control of cea. Deletion of this site resulted in a loss of the stimulatory effects of cAMP in a cya mutant.This publication has 16 references indexed in Scilit:
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