Abstract
Experiments have been made to elucidate the problem of hyperosmotic regulation in the mud-dwelling euryhaline amphipod,Corophium volutator.The animal produced urine hypo-osmotic to the blood when acclimatized to low salinities, and isosmotic urine at salinities above 20%. The restricted permeable areas of the cuticle have been localized by silver staining. Over a range of salinities from 1 to 35 %C. volutatorwas found to maintain Na+, K-−, Ca2+, Cl-−, more concentrated than the medium, and Mg2~less concentrated. The importance of these mechanisms in assisting hyperosmotic regulation is discussed.

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