DNA Probe for Lactobacillus delbrueckii
- 1 June 1990
- journal article
- research article
- Published by American Society for Microbiology in Applied and Environmental Microbiology
- Vol. 56 (6) , 1967-1970
- https://doi.org/10.1128/aem.56.6.1967-1970.1990
Abstract
From a genomic DNA library of Lactobacillus delbrueckii subsp. bulgaricus, a clone was isolated which complements a leucine auxotrophy of an Escherichia coli strain (GE891). Subsequent analysis of the clone indicated that it could serve as a specific DNA probe. Dot-blot hybridizations with over 40 different Lactobacillus strains showed that this clone specifically recognizes L. delbrueckii subsp. delbrueckii, bulgaricus, and lactis. The sensitivity of the method was tested by using an α- 32 P-labeled DNA probe.This publication has 16 references indexed in Scilit:
- Detection of specific sequences among DNA fragments separated by gel electrophoresisPublished by Elsevier ,2006
- DNA probe technology for rapid detection of Haemophilus influenzae in clinical specimensJournal of Clinical Microbiology, 1988
- Specific DNA Probe for the Diagnosis of Plasmodium falciparum MalariaScience, 1986
- Development of a DNA probe to detect Salmonella typhiJournal of Clinical Microbiology, 1985
- Detection of adenoviruses in stool specimens by nucleic acid spot hybridizationJournal of Medical Virology, 1985
- A DOT HYBRIDISATION ASSAY FOR DETECTION OF ROTAVIRUSThe Lancet, 1983
- Nonsense suppression in Schizosaccharomyces pombe: The S. pombe Sup3-e tRNA Ser UGA gene is active in S. cerevisiaeMolecular Genetics and Genomics, 1982
- Identification of DNA viruses by membrane filter hybridizationJournal of Clinical Microbiology, 1982
- Nucleic acid spot hybridization: rapid quantitative screening of lymphoid cell lines for Epstein-Barr viral DNA.Proceedings of the National Academy of Sciences, 1980
- Improved medium for lactic streptococci and their bacteriophages.1975