Synthetic muscle promoters: activities exceeding naturally occurring regulatory sequences

1 March 1999

journal article
research article
Published by Springer Nature in Nature Biotechnology

Vol. 17 (3) , 241-245
https://doi.org/10.1038/6981

Abstract

Relatively low levels of expression from naturally occurring promoters have limited the use of muscle as a gene therapy target. Myogenic restricted gene promoters display complex organization usually involving combinations of several myogenic regulatory elements. By random assembly of E-box, MEF-2, TEF-1, and SRE sites into synthetic promoter recombinant libraries, and screening of hundreds of individual clones for transcriptional activity in vitro and in vivo, several artificial promoters were isolated whose transcriptional potencies greatly exceed those of natural myogenic and viral gene promoters.

Keywords

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