A Modified Method for Preparing and Counting Viable Suspensions of Tubercle Bacilli

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Abstract
SUMMARY: A modified method derived from that of Fenner, Martin & Pierce (1949) is described for the preparation of homogeneous suspensions of single organisms of one strain of Mycobacterium tuberculosis at concentrations of about 108 single organisms per ml. A technique is also described for the accurate assessment of viable counts in a period of 14 days.