Identification of Histoplasma capsulatum from Culture Extracts by Real-Time PCR

Abstract
We designed and tested a real-time LightCycler PCR assay for Histoplasma capsulatum that correctly identified the 34 H . capsulatum isolates in a battery of 107 fungal isolates tested and also detected H . capsulatum in clinical specimens from three patients that were culture positive for this organism.