Automated Purification of Human Protein Band 3, The Major Integral Protein of the Erythrocyte Membrane
- 31 August 1988
- journal article
- research article
- Published by Taylor & Francis in Preparative Biochemistry
- Vol. 18 (3) , 293-301
- https://doi.org/10.1080/00327488808062530
Abstract
Human erythrocyte protein band 3 was purified from a Triton X-100 extract of white ghosts. This purification, including an ion-exchange chromatography and a group-affinity chromatography, was automated. The apparatus was assembled from commercially available elements and allowed for the recovery of 2 to 3 mg pure band 3 in 2 hr. The purification could be repeated several times a day. The advantages of automation are discussed.Keywords
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