CHRONIC INTRAVENOUS-INFUSION IN THE RAT - NON-SURGICAL APPROACH

  • 1 January 1979
    • journal article
    • research article
    • Vol. 29  (1) , 82-84
Abstract
A rapid, simplified technique of i.v. catheterization of the unanesthetized rat was developed for continuous infusion. A 19-gauge thin-walled needle was inserted percutaneously into the dilated tail vein, and polyethylene tubing was advanced through the needle into the vein. The catheter was protected by wire mesh electrical sheathing taped securely to the tail. This approach eliminated the need for anesthesia and surgery which were traumatic to the animal and time consuming for the investigator. This method was used successfully in 32 rats given continuous infusion for up to 5 days. In many experimental settings chronic intravenous administration of drugs, hormones or other agents is required. To evaluate the efficacy of various drugs in the treatment of oxidant lung injury, a simple means for continuous infusion in a large number of rats was sought.