Abstract
An appreciative amount of crossing over in the 4th chromosome of diplo-IV triploid females of D. melanogaster has made possible a building up to multiple recessive stocks in four-point test-cross experiments. The mutant alleles, ci, gvl, eyR and svna, were used and the results show that the true sequence is ci, gvl, bt, ey, and sv with the possibilities of ci and gvl reversed because unexpected cross over classes occur. Contrary to Bridges and Brehme, but in conformity with Dubinin, Sokolov and Tiniakov, the white-mottled-5 when tested over ci shows a marked position effect on dominance of ci.

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