A Comparison of Two Methods of Ashing Enamel and Dentin

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Abstract
About 50 teeth were separated into enamel and dentin. An aliquot of each tissue was then ashed by incineration (heat ash) and another by extraction with 3% KOH in ethylene glycol (glycol ash). Ca, Mg and P analyses were made on every ashed aliquot. Incineration drives off CO2 so that the heat ash always was a smaller percentage of the dry wt. than was the glycol ash, which contained all of the CO2. The percentages of Ca Mg and P in the heat ash were therefore higher than in the glycol ash.