α-Amylase production in high cell density submerged cultivation of Aspergillus oryzae and A. nidulans
- 1 January 2001
- journal article
- short contribution
- Published by Springer Nature in Applied Microbiology and Biotechnology
- Vol. 55 (1) , 81-84
- https://doi.org/10.1007/s002530000456
Abstract
The effect of biomass concentration on the formation of Aspergillus oryzaeα-amylase during submerged cultivation with A. oryzae and recombinant A. nidulans strains has been investigated. It was found that the specific rate of α-amylase formation in chemostats decreased significantly with increasing biomass concentration in the range of approx. 2–12 g dry weight kg−1. When using a recombinant A. nidulans strain in which the gene responsible for carbon catabolite repression of the A. oryzaeα-amylase gene (creA) was deleted, no significant decrease in the specific rate of α-amylase formation was observed. On the basis of the experimental results, it is suggested that the low value of the specific α-amylase productivity observed at high biomass concentration is caused by slow mixing of the concentrated feed solution in the viscous fermentation medium.Keywords
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