Use of Centrifugal Force to Promote Adsorption of Myxoma Virus to Cell Monolayers.

Publisher Website
Google Scholar
Abstract
Centrifugation of myxoma virus onto preformed monolayers of rabbit kidney and rabbit heart fibroblast cells in one oz. presciption bottles substantially increased the number of plaque-forming units of virus detected as compared to other methods of adsorption. About 50% more plaque-forming units were detected after centrifugation than with a technique that continually redistributed the inoculum over the cell monolayer for 4 hours. The maximum number of plaque-forming units was obtained after the plaque bottles were centrifuged in a basket rotor (11" in diameter) for only 15 minutes at 1900 g at room temperature. Centrifuging a low multiplicity of myxoma virus at 1270 g for 10-20 minutes onto rabbit kidney cells growing as a monolayer on the bottom of shell vials (22 mm. in diameter) was sufficient to sediment enough virus to result in the infection of all the cells.