Lysis of chromaffin granules by phospholipase A2‐treated plasma membranes
- 17 February 1986
- journal article
- Published by Wiley in FEBS Letters
- Vol. 196 (2) , 349-352
- https://doi.org/10.1016/0014-5793(86)80277-0
Abstract
The possible involvement of phospholipase A2 (PLA2) in the release of catecholamines was examined in a cell-free model system, using isolated chromaffin granules and plasma membranes of adrenal medulla cells. Plasma membranes treated with PLA2 in the presence of Ca2+ caused lysis of chromaffin granules which was also dependent on Ca2+. This finding suggests that Ca2+ acts in two steps of exocytosis, namely in the transformation of plasma membranes into a lytic form by PLA2+ and in the interaction of chromaffin granules with plasma membranes. These findings show good agreement with recently reported findings in leaky adrenal medulla cells, and suggest the involvement of PLA2+ in the release of catecholamines.Keywords
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