RECONSTITUTION OF CATECHOLAMINE-SENSITIVE ADENYLATE-CYCLASE ACTIVITY - INTERACTION OF COMPONENTS FOLLOWING CELL-CELL AND MEMBRANE-CELL FUSION
- 1 January 1977
- journal article
- research article
- Vol. 3 (4) , 227-238
Abstract
Heterokaryons formed by the fusion of adenylate cyclase-deficient [murine lymphoma] S49 cells and .beta.-adrenergic receptor-deficient [murine L] B82 cells in the presence of cycloheximide display a catecholamine-sensitive adenylate cyclase activity. Similar complementation can be observed when receptor-replete membranes from the cyclase-deficient cell are fused with intact B82 cells. Using the cell fusion technique it can be demonstrated that the uncoupled S49 cell variant has a functional .beta.-adrenergic receptor but that this variant and the adenylate cyclase-deficient variant are not complentary. Hypothetically, both clones may share a common defect in regulatory components of adenylate cyclase or both may lack a specific coupling factor.This publication has 6 references indexed in Scilit:
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