Low temperature microspectrofluorometry: design of a ‘cold chamber‘
- 1 January 1978
- journal article
- research article
- Published by Wiley in Journal of Microscopy
- Vol. 112 (1) , 95-101
- https://doi.org/10.1111/j.1365-2818.1978.tb01157.x
Abstract
Low temperature microspectrofluorometry allows an improvement of spectral resolution and an increase of fluorescence intensity. Suppression of fluorescence fading, or at least a marked reduction, is also obtained. A cooling chamber for microspectrofluorometric measurement is described which allows the cooling, under a microscope, of a biological sample down to liquid nitrogen temperature. Objectives with numerical apertures better than 1.0 and a magnification power up to 100 × can be used. Low temperature measurements on a histological sample are presented and discussed.This publication has 7 references indexed in Scilit:
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