ACTIN REARRANGEMENT IN LIVING CELLS REVEALED BY MICRO-INJECTION OF A FLUORESCENT PHALLOIDIN DERIVATIVE
- 1 January 1981
- journal article
- research article
- Vol. 24 (2) , 176-183
Abstract
A fluorescent derivative of phalloidin with a high affinity for F-actin was microinjected into tissue culture cells [rat mammary cell and rat kangaroo kidney PtK2 cell] and its intraceullular reorganization was followed by television image intensification and video recording. When the F-actin stabilizing drug is used at concentrations which do not inhibit cellular movement, rearrangement of fluorescently labeled microfilament bundles can be followed directly. The possibilities that active ruffles are governed by structural rules, different from those applied to stress fibers and that actin may be released from microfilaments in a form different from G-actin are discussed.This publication has 23 references indexed in Scilit:
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