The Effects of Insect Hormones on the Multiplication Rates of Cultured Insect Cells in Vitro

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Abstract
The effects were examined of some insect hormones on the multiplication rates of cells in cultures obtained from Antheraea eucalypti pupal ovarioles. 20-hydroxyecdysone and ponasterone A showed stimulating effects on cell multiplication at the concentration of 0.1 μg per ml. Proteinic brain hormone, and farnesol had no stimulating effect on the cell growth. At the concentration of 10.0 μg per ml, 20-hydroxyecdysone, ponasterone A and farnesol impaired the cell growth markedly if no protein was present in the culture media, but this deleterious effect was moderated in each instance by the addition of bovine plasma albumin to the culture medium. Combinations of 20-hydroxyecdysone and farnesol at various ratios caused no stimulation of the cell multiplication.