AGGLUTINATION OF BLADDER CELLS BY CONCANAVALIN-A DURING THE EARLY PHASE OF TREATMENT OF RATS WITH N-BUTYL-N-(4-HYDROXYBUTYL)NITROSAMINE

  • 1 January 1979
    • journal article
    • research article
    • Vol. 39  (9) , 3353-3356
Abstract
N-butyl-N-(4-hydroxybutyl)nitrosamine was given to male Wistar rats at a dose of 0.05% in the drinking water for 1-5 wk, and agglutination of cells isolated from their bladder by concanavalin A (Con A) was determined at intervals during and after treatment. Mucosal cells were isolated from everted bladder by EDTA treatment and sonication. As early as 1 wk after the start of treatment, Con A caused some agglutination of isolated bladder cells, and this agglutination increased with time, reaching an almost constant value from the 3rd week. Con A agglutination of bladder cells induced by N-butyl-N-(4-hydroxybutyl)nitrosamine treatment for only 1 wk appeared to be irreversible, and it was still observed 2 wk after the end of treatment. Scanning electron microscopy showed that microvilli developed on the luminal surface of mucosal cells in situ at the time when the isolated cells became agglutinable with Con A. Measurement of agglutinability of isolated bladder cells with Con A might be a useful way of detecting very early changes in bladder carcinogenesis.