Induction of Cytolysis of Cultured Lymphoma Cells by Adenosine 3′:5′-Cyclic Monophosphate and the Isolation of Resistant Variants
- 1 January 1973
- journal article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 70 (1) , 76-79
- https://doi.org/10.1073/pnas.70.1.76
Abstract
Cultured mouse lymphosarcoma cells are killed on exposure to 0.1 mM N 6 , O 2′ -dibutyryl-adenosine 3′:5′-cyclic monophosphate. A population of cells resistant to the killing effect of dibutyryl cyclic AMP at concentrations as high as 1 mM was selected. The growth characteristics of the resistant cells were similar to those of the sensitive parental line. However, the resistant cells contain less cytoplasmic cyclic AMP-binding proteins and decreased cyclic AMP-stimulated protein kinase activity. It is proposed that transition from sensitivity to resistance to dibutyryl cyclic AMP in lymphoma cells is connected with a modification of the cyclic AMP-binding protein, which appears to be the regulatory subunit of the cyclic AMP-activated protein kinase.Keywords
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