A rapid method for measuring production of yellow rust spores on single seedlings to assess differential interactions of wheat cultivars with Puccinia striiformis

Publisher Website
Google Scholar
Abstract
SUMMARY: Uredospore production of Puccinia striiformis in single wheat seedlings was assessed by weighing spores on an electrical microbalance, by counting on a haemocytometer or by measuring turbidity of spore suspensions with a spectrophotometer and compared with production from groups of seedlings determined by weighing spores on an analytical balance. The data were used to assess differential interaction of wheat cultivars Hybrid 46 and Joss Cambier with two isolates of race 104 E137 of P. striiformis and cvs Maris Templar and Joss Cambier with two isolates of race 41 E136. A significant differential interaction was shown in both experiments by each method but most rapidly and with the minimum of materials by the single‐plant microbalance technique. Measurements of spore production demonstrated differences between isolates within races more clearly than the conventional visual assessment of yellow rust symptoms.