INVESTIGATIONS INTO THE QUANTITATIVE DETERMINATION OF ANTIHORMONES AGAINST PREGNANT MARES' SERUM HORMONE

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Abstract
To throw light on the quantitative aspect of the hormone-antihormone reactions, 574 immature female rats were injd. subcut. with mixtures of pregnant mares'' serum gonadotropin and a rabbit antiserum against this hormone. The avg. uterine and ovarian wt. obtained in rats treated with various combinations of hormone and antiserum were compared with the dose-response curves obtained in animals treated with the hormone alone. From these curves the amt. of hormone neutralized by the antiserum was calculated. It was found that the antigonadotropic activity of a serum depends on the ratio between the amt. of serum and that of gonadotropin, the small doses of serum being relatively much more effective than larger quantities. Any statement concerning the "titer" of an antiserum is therefore of little value.

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