1,25-DIHYDROXYVITAMIN-D3 RECEPTORS IN HUMAN EPITHELIAL CANCER CELL-LINES
- 1 January 1982
- journal article
- research article
- Vol. 42 (3) , 856-859
Abstract
Specific, high-affinity cytosolic receptors for 1,25-dihydroxyvitamin D3 were demonstrated in 5 human cancer cell lines [HS0578T breast cancer cells, A549 lung carcinoma cells, HUK-HeLa cervical carcinoma cells and melanomas G-361 and HS695T cells]. Binding affinity (Kd) of the receptors for 1,25-dihydroxyvitamin D3 were all .apprx. 0.2 nM, and receptor content ranged from 21-174 fmol/mg cytosol protein. The receptors from all 5 cell lines sedimented at 3.2S on sucrose density gradients and exhibited preferential affinity for 1,25-dihydroxyvitamin D3 compared to other vitamin D metabolites.This publication has 17 references indexed in Scilit:
- Unoccupied 1,25-dihydroxyvitamin D3 receptors. Nuclear/cytosol ratio depends on ionic strength.Journal of Biological Chemistry, 1980
- Characterization of the 1 alpha,25-dihydroxyvitamin D3 receptor from rat intestinal cytosol.Journal of Biological Chemistry, 1979
- Characterization of a cytoplasmic receptor-like binder for 1 alpha, 25-dihydroxycholecalciferol in rat intestinal mucosa.Journal of Biological Chemistry, 1979
- SPECIFIC CYTOSOL RECEPTORS FOR 1,25-DIHYDROXYVITAMIN D3IN HUMAN INTESTINEJournal of Clinical Endocrinology & Metabolism, 1979
- Enhancement of Heterotransplanted Human Tumor Graft Survival in Nude Mice Treated With Antilymphocyte Serum and in Congenitally Athymic-Asplenic (lasat) Mice2JNCI Journal of the National Cancer Institute, 1978
- Basic and Clinical Concepts Related to Vitamin D Metabolism and ActionNew England Journal of Medicine, 1977
- Basic and Clinical Concepts Related to Vitamin D Metabolism and ActionNew England Journal of Medicine, 1977
- Specific cytosol-binding protein for 1,25-dihydroxyvitamin D3 in rat intestine.Journal of Biological Chemistry, 1977
- Two Syngeneic Cell Lines from Human Breast Tissue: The Aneuploid Mammary Epithelial (Hs578T) and the Diploid Myoepithelial (Hs578Bst) Cell Lines 2JNCI Journal of the National Cancer Institute, 1977
- A Rapid and Sensitive Method for the Quantitation of Microgram Quantities of Protein Utilizing the Principle of Protein-Dye BindingAnalytical Biochemistry, 1976