Biological control ofVerticillium dahliaekleb. by natural occurring rhizosphere bacteria

Abstract
Three bacteria were isolated from the rhizosphere of Brassica napus L. and Capsella bursa‐ pastoris (L.) MED. and determined as Bacillus subtilis, Pseudomonas fluorescens and Stenotrophomonas maltophilia. The efficacy of the bacterial antagonists against different phytopathogenic fungi was proven by using in vitro bioassays. Antifungal mechanisms (production of siderophores, of lytic enzymes and of antibiotics) of these isolates were also investigated with positive results. The efficiency of the bacteria as biocontrol agents against Verticillium dahliae KLEB. on Brassica napus L. was tested in greenhouse and field experiments. The results indicated that Stenotrophomonas maltophilia was the best biocontrol agent against Verticillium‐wilt of oilseed rape. These isolate showed the highest efficacy under in vitro and in vivo conditions.