A strategy simplifying site-directed mutagenesis in the CD4-binding region of HIV gp 120
- 31 August 1989
- journal article
- research article
- Published by Springer Nature in Archiv für die gesamte Virusforschung
- Vol. 107 (3-4) , 301-305
- https://doi.org/10.1007/bf01317925
Abstract
By site-directed mutagenesis, two unique restriction sites, surrounding the CD4-binding DNA region of HIV gp 120, were created. The mutations do not cause amino acid substitutions but generate a DNA fragment, which is stable when cloned into M13 for further mutagenesis and is also easy to reclone.This publication has 8 references indexed in Scilit:
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