Purification of L-Isoleucyl t-rna Synthetase by Affinity Chromatography
Open Access
- 1 January 1974
- journal article
- research article
- Published by Taylor & Francis in Preparative Biochemistry
- Vol. 4 (3) , 227-241
- https://doi.org/10.1080/00327487408068200
Abstract
L-Isoleucyl t-RNA synthetase was purified to 97% purity in a single step by affinity chromatography on a column carrying L-isoleucinyl 5′-adenylate as the insolubilized ligand. Recovery was 50–60%.Keywords
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