Cytometric analysis of small lymphocytes in the bone marrow and lymphatic tissues in neonatally thymectomized and nude mice, with particular reference to differentiation of B-lymphocytes.

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Abstract
In neonatally thymectomized mice and nude mice, small lymphocytes in the bone marrow, Peyer''s patches, lymph nodes and spleen were studied qualitatively and quantitatively by EM. Marrow lymphocytes which were mostly small lymphocytes were classified into dark and light types. The greater proportion of marrow small lymphocytes were of the dark type. Small lymphocytes in Peyer''s patches, lymph nodes and spleen were of the light type. Nuclear and cellular sizes of small lymphocytes were estimated stereologically. The nucleus-cell ratio, volumetric proportions of mitochondria, Golgi apparatus, tubular complexes and dense bodies to the cell body and proportions of nucleoli and nuclear bodies to the nucleus were also determined. Quantitative results thus obtained suggested that light, small lymphocytes in the bone marrow were cytometrically similar to small lymphocytes in Peyer''s patches, lymph nodes and spleen and that dark, small lymphocytes in the bone marrow were different from other small lymphocytes. The findings were discussed in particular relation to morphological differentiation of B[bone marrow-derived]-lymphocytes.

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