MESSAGE AMPLIFICATION PHENOTYPING (MAPPING) - A TECHNIQUE TO SIMULTANEOUSLY MEASURE MULTIPLE MESSENGER-RNAS FROM SMALL NUMBERS OF CELLS

  • 1 November 1989
    • journal article
    • research article
    • Vol. 7  (10) , 1096-1103
Abstract
A rapid and highly sensitive technique (MAPPing: message amplification phenotyping) has been developed to simultaneously analyze the array of messenger RNAs made by small numbers of cells. The technique incorporates a micro-procedure for isolating RNA, reverse transcription of total cellular RNA to produce cDNA, and enzymatic amplification of cytokine-specific DNA fragments using the polymerase chain reaction. In this study, the technique has been applied to the analysis of cytokines produced by lymphoid cells ranging in number from a single cell to 106 cells. The technique should be applicable to virtually any tissue or cell type.