Duplex to quadruplex equilibrium of the self‐complementary oligonucleotide d(GGGGCCCC)

Abstract
The structure of the deoxyaligonucleotide d(GGGGCCCC) has been monitored by 1H and 31 P NMR, and by gel electrophoresis. In low‐salt solution, this oligonucleotide forms a stable duplex structure. Upon titration with KCl, an equilibrium is established between duplex and quadruplex forms. The quadruplex form is the dominant one at physiological KCl concentrations, despite the fact that fewer hydrogen bonds are formed per strand in the quadruplex than in the duplex. © 1995 John Wiley & Sons, Inc.