Thin-layer Chromatography of Plant Extracts. II. Sequential Extraction of Plant Material.

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Abstract
Extraction of plant material with a series of solvents of increasing dielectric constant provides only a rough and partial separation of the substances. A large proportion of the constituents appear in several extracts, and each fraction includes substances with polar properties so different that in thin-layer chromatography some substances remain at the starting-point even if others are moving close to the front.